Publications

Select publications from the laboratory

  • Nazy I, Kelton JG, Moore JC, Clare R, Horsewood P, Smith JW, Ivetic N, D’Souza V, Li N, Arnold DM. Autoantibodies to thrombopoietin and the thrombopoietin receptor in patients with immune thrombocytopenia. Br J Haematol, 2018. [in press]
  • Warkentin TE, Arnold DM, Kelton JG, Sheppard JI, Smith JW, Nazy I. Platelet-activating antibodies are detectable at the earliest onset of heparin-induced thrombocytopenia, with implications for the operating characteristics of the serotonin-release assay. Chest 2018 Jan 8. [Epub ahead of print]
  • Arnold DM, Nazy I, Clare R, Jaffer A, Aubie B, Li N, Kelton JG. Misdiagnosis of primary immune thrombocytopenia and frequency of bleeding: lessons from the McMaster ITP Registry. Blood Adv. 2017 Nov 28; 1(25): 2414-2420.
  • Huynh A, Arnold DM, Moore JC, Smith JW, Kelton JG, Nazy I. Development of high-yield expression and purification system for platelet factor 4. Platelets 2017 Nov 27: 1-8
  • Arnold DM, Vrbensky Jr, Karim N, Smith JW, Liu Y, Ivetic N, Kelton JG, Nazy I. The effect of rituximab on anti-platelet autoantibody levels in patients with immune thrombocytopenia. Br J Haematol. 2017 Jul; 178(2): 302-307.
  • Warkentin TE, Sheppard JI, Linkins LA, Arnold DM, Nazy I. Performance characteristics of an automated latex immunoturbidimetric assay (Hemosil HIT- Ab (PF4H) for the diagnosis of immune heparin induced thrombocytopenia. Thromb Res. 2017 May; 153:108-117.
  • Staibano P, Arnold DM, Bowdish DM, Nazy I. The unique immunological features of heparin-induced thrombocytopenia. Br J Hematol. 2017 Apr; 177(2): 198-207.
  • Solh Z, Breakey V, Murthy P, Smith JW, Arnold DM. Triplets with neonatal alloimmune thrombocytopenia due to antibodies against human platelet antigen 1a. Transfusion. 2016 May; 56(5): 1168-1170.
  • Ivetic N, Nazi I, Karim N, Clare R, Smith JW, Moore JC, Hope KJ, Kelton JG, Arnold DM. Producing megakaryocytes from a human peripheral blood source. Transfusion. 2016 May; 56(5): 1066-74.
  • Nazi I, Arnold DM, Warkentin TE, Smith JW, Staibano P, Kelton JG. Distinguishing between anti-platelet factor 4/heparin antibodies that can and cannot cause heparin-induced thrombocytopenia. J Thromb Haemost. 2015 Oct 13; 13(10): 1900-7.
  • Warkentin TE, Arnold DM, Nazi I, Kelton JG. The platelet serotonin-release assay. Am J Hematol. 2015 Jun; 90(6):564-72.
  • Arnold DM, Nazi I, Toltl L, Ross C, Ivetic N, Smith J, Yang L, Kelton, J. Antibody binding to megakaryocytes in vivo in patients with immune thrombocytopenia. Eur J Hematology 2015 Feb 13.
  • Arnold DM, Curtis BR, Bakchoul T; The Subcommittee of Platelet Immunology of the Scientific and Standardization Committee of the International Society on Thrombosis and Haemostasis. Recommendations for standardization of laboratory testing for drug-induced immune thrombocytopenia: communication from the SSC of the ISTH. J Thromb Haemost. 2015 Apr; 13(4):676-8.
  • Bakchoul T, Heckmann,, Arnold DM, Paes B, Bassler D, Theiele T, Smith JW, Volker K. Management of infants born with severe neonatal alloimmune thrombocytopenia. Transfusion 2014 Mar; 54 (3): 640-5.
  • Nazi I, Kelton JG, Larche M, Snider DP, Heddle NM, Crowther M, Cook RJ, Tinmouth AT, Mangel J, Arnold DM. The effect of rituximab on vaccine responses in patients with immune thrombocytopenia. Blood. 2013 Sep 12; 122(11): 1946-53.
  • Arnold DM, Nazi I, Warkentin TW, Smith JW, Toltl LJ, George JN, Kelton JG. Approach to the diagnosis and management of drug-induced immune thrombocytopenia. Transfusion Medicine Reviews 2013 Jul; 27(3): 137-45.
  • Nazi I, Arnold DM, Smith JW, Horsewood P, Moore JC, Warkentin TE, Crowther MA, Kelton JG. FcyRlla proteolysis as a diagnostic biomarker for heparin-induced thrombocytopenia. J Throm Haemost 2013 Jun; 11(6):1146-53.
  • Mahabir VK, Ross C, Popovic S, Sur ML, Bourgeois J, Lim W, George JN,Wang G, Cook RJ, Toltl LJ, Nazi I, Kelton JG, Arnold DM. A Blinded Study of Bone Marrow Examinations in Patients with Primary Immune Thrombocytopenia. Eur J Haematol. 2013 Feb; 90 (2): 121-6.
  • Arnold DM, Kukaswadia S, Nazi I, Esmail A, Dewar L, Smith JW, Warkentin TE, Kelton JG. A systematic evaluation of laboratory testing for drug-induced immune thrombocytopenia. J Thromb Haemost. 2013 Jan; 11(1):169-76.
  • Kelton JG, Warkentin TE, Moore JC, Arnold DM, Nazi I, Arepally GM, Roach JM Fier I. A prospective study measuring the development of antibodies against platelet factor 4 in healthy males following exposure to heparins. J Thromb Haemost 2012 Jul; 10(7):1446-9.
  • Arnold DM, Santoso S. Greinacher A, on behalf of the Platelet Immunology Scientific Subcommittee of ISTH. Recommendations for the implementation of platelet autoantibody testing in clinical trials of immune thrombocytopenia. J Thromb Haemost. 2012 Apr;10 (4):695-7.
  • Toltl LJ, Nazi I, Jafari R, Arnold DM. Piecing together the humoral and cellular mechanisms of immune thrombocytopenia. Sem Thromb Haem 2011 Sept; 37(6): 631-9.
  • Nazi I, Smith JW, Arnold DM, Horsewood P, Moore JC, Warkentin TE, Kelton JG. The association between platelet activation and FcyRlla proteolysis. J Thromb Haemost 2011 Apr; 9(4):885-7.
  • Arnold DM, Nazi I, Kelton JG. New treatments for idiopathic thrombocytopenic purpura: rethinking old hypotheses. Expert Opin Investig Drugs. 2009 June 18(6):805-819.
  • Arnold DM, Bernotas A, Nazi I, Stasi R, Kuwana M, Liu Y, Kelton JG and Crowther MA. Platelet Count response to H.pylori treatment in patients with Immune Thrombocytopenic Purpura with and without H. pylori infection: a systematic review. Haematologica 2009 June; 94(6):850-856
  • Smith JW. Platelet antibody testing in Ontario: the right test at the right time. Advocate, Ontario Society Medical Technologists 16:2, 21-22, 2009 Feature Article
  • Arnold DM, Smith JW, Kelton JG. Diagnosis and Management of Neonatal Alloimmune Thrombocytopenia. Transfusion Medicine Reviews 2008 Oct; 22(4):255-67.
  • Moore JC, Arnold DM, Warkentin TE, Warkentin AE, Kelton JG. An algorithm for resolving "indeterminate" test results in the platelet serotonin release assay for investigation of heparin-induced thrombocytopenia. J Thromb Haemost. 2008; 6(9):1595-7.
  • Moore JC, Arnold DM, Leber BF, Clare R, Molnar GJ, Kelton JG. Intravenous Immunoglobulin as an Adjunct to Plasma Exchange for the Treatment of Chronic Thrombotic Thrombocytopenic Purpura. Vox Sang. 2007 Aug; 93(2):173-5.
  • Arnold DM, Kelton JG. Testing for heparin-induced thrombocytopenia: are we there yet? J Thromb Haemost. 2007 Jul; 5(7):1371-2.
  • Arnold DM, Kelton JG. Heparin-induced thrombocytopenia: an iceberg rising. Mayo Clin Proc 2005 Aug; 80(8):988-90.
  • Metcalfe P, Watkins NA, Ouwehand WH, Kaplan C, Newman P, Kekomaki R, deHaas M, Aster R, Shibata Y, Smith J, Kiefel V, Sanotso S. Nomenclature of human platelet antigens. Vox Sanq 85:240-5, 2003.
  • Shannon A, Smith JW, Nagel K., Levesque R., Warkentin TE, Barr R. Selective thrombocytopenia in children with Wilms tumor: An immune-mediated effect of actinomycin D. Med Pediatr Oncol 41:483-5, 2003
  • West KA, Anderson DR, McAlister VC, Hewlett TJC, Belitsky P, Smith JW, Kelton JG. Alloimmune thrombocytopenia after organ transplantation. N Eng J Med 341:1504-1507, 1999
  • Kelton JG, Smith JW, Moffatt D, Santos A, Horsewood P. The interaction of ancrod with human platelets. Platelets 10:24-29, 1999
  • Kelton JG, Smith JW, Horsewood P, Warner MN, Warkentin TE, Finberg RW, Hayward CPM. ABH antigens on human platelets: expression on the glycosyl phosphatidyl inositol-anchored protein, CD109. J Lab Clin Med 132:142-8, 1998
  • Bordin JO, Kelton JG, Warner MN, Smith JW, Hayward CPM, Warkentin TE, Denomme GA. Maternal alloimmunization to the Gov alloantigens on human platelets. Transfusion 37: 823-28, 1997
  • Warkentin TE, Smith JW. The Alloimmune Thrombocytopenic Syndromes. Transfus Med Rev, 1997 Review
  • Denomme G, Horsewood P, Xu W, Smith J, Kelton J. A simple and rapid competitive enzyme linked immunosorbent assay to identify HPA-1a (PlA1)-negative donor platelet units. Transfusion 36:805-808, 1996
  • Smith JW, Hayward CPM, Horsewood P, Warkentin TE, Denomme GA, Kelton JG. Characterization and localization of the Gov-a/b alloantigens to the glycosyl-phosphatidylinositol-anchored protein CDw109 on human platelets. Blood 86:2807-14, 1995
  • Denomme GA, Waye JS, Burrows RF, Hayward CPM, Warkentin TE, Horsewood P, Smith JW, Jelsema RD, Zuidema LJ, Kelton JG. The prenatal identification of fetal incompatibility in neonatal alloimmune thrombocytopenia using amniotic fluid and variable number of tandem repeats (VNTR) analysis. Br J Haematol 91:742-46, 1995
  • Bordin JO, Smith JW, Hayward CPM, Warkentin TE, Kelton JG. Bacterial infection-associated improvement of platelet counts in two patients with chronic and unresponsive idiopathic thrombocytopenic purpura with normal platelet survival studies. Br J Haematol 90:332-35, 1995
  • Kelton JG, Smith JW, Warkentin TE, Hayward CPM, Horsewood P. Immunoglobulin G from patients with heparin-induced thrombocytopenia binds to a complex of heparin and platelet factor 4. Blood 83:3232-39, 1994
  • Warkentin TE, Hayward CP, Boshkov LK, Santos AV, Sheppard JA, Bode AP, Kelton JG. Sera from patients with heparin-induced thrombocytopenia generate platelet-derived microparticles with procoagulant activity: an explanation for the thrombotic complications of heparin-induced thrombocytopenia. Blood 84:3691-9, 1994
  • Smith JW, Hayward CPM, Warkentin TE, Horsewood P, Kelton JG. Investigation of human platelet alloantigens and glycoproteins using non-radioactive immunoprecipitation. J Immunol Methods 158:77-85, 1993
  • Hayward CPM, Bainton DF, Smith JW, Horsewood P, Kelton JG. Multimerin is found in the alpha-granules of resting platelets and is synthesized by a megakaryocytic cell line. J Clin Invest 91:2630-2639, 1993
  • Horsewood P, Smith JW, Kelton JG. Monoclonal antibodies against platelet glycoproteins. Transfus Med Rev 7:156-172, 1993 Review
  • Burrows RF, Kelton JG. Fetal thrombocytopenia and its relation to maternal thrombocytopenia. N Engl J Med 329:1436-6, 1993
  • Warkentin TE, Smith JW, Hayward CPM, Ali AM, Kelton JG. Thrombocytopenia caused by passive transfusion of anti-glycoprotein la/lla alloantibody (anti-HPA-5b). Blood 79:2480-2484, 1992
  • Sinha RK, Santos AV, Smith JW, Horsewood P, Andrews M, Kelton JG. Rabbit platelets do not express Fc receptors for IgG. Platelets 3:35-39, 1992
  • Denomme GA, Smith JW, Kelton JG, Bell DA. A human monoclonal autoantibody to platelet glycoprotein IIb derived from normal human lymphocytes. Blood 79:447-451, 1992
  • Hayward CPM, Warkentin TE, Horsewood P, Smith JW, Kelton JG. P-155, a multimeric protein that is expressed on activated platelets. J Biol Chem 266:7114-7120, 1991
  • Kelton JG, Smith JW, Horsewood P, Humbert JR, Warkentin TE. The Gov-a/b alloantigen system on human platelets. Blood 75:2172-2176, 1990
  • Burrows RF, Kelton JG. Thrombocytopenia at delivery: a prospective survey of 6715 deliveries. Am J Obstet Gynecol 162:173-4, 1990
  • Moore J, Murphy WG, Kelton JG. Calpain proteolysis of von Willebrand factor enhances its binding to platelet membrane glycoprotein IIb/IIIa: an explanation for platelet aggregation in thrombotic thrombocytopenic purpura. Br J Haemaol 74:457-64, 1990
  • Burrows RF, Kelton JG. Alloimmune neonatal thrombocytopenia associated with incidental maternal thrombocytopenia. Am J Hematol 35:43-4, 1990
  • Smith JW, Kelton JG, Horsewood P, Brown C, Giles A, Hunter D, Meyer R, Woods V, Burrows R. Platelet-specific alloantigens on the platelet glycoprotein la/lla complex. Br J Haematol 72:534-38, 1989
  • Kelton JG, Murphy WG, Lucarelli A, Garvey-Williams J, Santos A, Meyer R, Powers P. A prospective comparison of four techniques for measuring platelet-associated IgG. Br J Haematol 71:97-105, 1989
  • Kelton JG, Sheridan D, Santos A, Smith JW, Steeves K, Smith C, Brown C, Murphy WG. Heparin-induced thrombocytopenia: Laboratory studies. Blood 72:925-30, 1988
  • Kelton JG, Smith JW, Santos AV, Murphy WG, Horsewood P. Platelet IgG Fc receptor. Am J Hematol 25:299-310, 1987
  • Murphy WG, Moore JC, Barr RD, Pai MK, Kelton JG. Relationship between platelet aggregating factor and von Willebrand factor in thrombotic thrombocytopenic purpura. Br J Haematol 66:509-13, 1987
  • Arnott J, Horsewood P, Kelton JG. Measurement of platelet-associated IgG in animal models of immune and nonimmune thrombocytopenia. Blood 69:1294-9, 1987
  • Kelton JG, Moore JC, Murphy WG. Studies investigating platelet aggregation and release initiated by sera from patients with thrombotic thrombocytopenic purpura. Blood 69:924-8, 1987
  • Sheridan D, Carter C, Kelton JG. A diagnostic test for heparin-induced thrombocytopenia. Blood 67:27030, 1986
  • Kelton JG, Sheridan D, Neame PB, Simon GT. Platelet fragments do not contribute to elevated levels of platelet associated IgG. Br J Haematol 61:707-15, 1985
  • Kelton JG, Denomme G, Lucarelli A, Garvey J, Powers P, Carter C. Comparison of the measurement of surface or total platelet-associated IgG in the diagnosis of immune thrombocytopenia. Am J Hematol 18:1-5, 1985
  • Kelton JG, Moore J, Santos A, Sheridan D. The detection of a platelet-agglutinating factor in thrombotic thrombocytopenic purpura. Ann Intern Med 101:589-93, 1984
  • Denomme G, Kelton JG. The production of platelet controls for assays quantitating platelet-associated IgG. Transfusion 23:516-8, 1983
  • Kelton JG, Steeves K. The amount of platelet-bound albumin parallels the amount of IgG on washed platelets from patients with immune thrombocytopenia. Blood 62:924-7, 1983
  • Kelton JG, Denomme G, Walker C, Horsewood P, Gauldie J. The measurement of platelet-associated IgG using an immunoradiometric assay. J Immunoassay 4:65-82, 1983
  • Kelton JG, Powers PJ, Carter CJ. A prospective study of the usefulness of the measurement of platelet-associated IgG for the diagnosis of idiopathic thrombocytopenic purpura. Blood 60:1050-3, 1982
  • Kelton JG, Denomme G. The quantitation of platelet-associated IgG on cohorts of platelets separated from healthy individuals by buoyant density centrifugation. Blood 60:136-9, 1982
  • Kelton JG, Meltzer D, Moore J, Giles AR, Wilson WE, Barr R, Hirsh J, Neame PB, Powers PG, Walker I, Bianchi F, Carter CJ. Drug-induced thrombocytopenia is associated with increased binding of IgG to platelets both in vivo and in vitro. Blood 58:524-9, 1981
  • Kelton JG, Moore J, Gauldie J, Neame PB, Hirsh J, Tozman E. The development and application of a serum assay for platelet-bindable IgG (S-PBIgG). J Lab Clin Med 98:272-9, 1981
  • Kelton JG, Blanchette VS, Wilson WE, Powers P, Pai KR, Effer SB, Barr RD. Neonatal thrombocytopenia due to passive immunization: prenatal diagnosis and distinction between maternal platelet alloantibodies and autoantibodies. N Engl J Med 303:1401-3, 1980
  • Kelton JG, Giles AR, Neame PB, Powers P, Hageman N, Hirsch J. Comparison of two direct assays for platelet-associated IgG (PAIgG) in assessment of immune and nonimmune thrombocytopenia. Blood 55:424-9, 1980
  • Kelton JG, Neame PB, Gauldie J, Hirsch J. Platelet-associated IgG in septicemia. N Engl J Med 301:272, 1979
  • Kelton JG, Neame PB, Gauldie J, Hirsh J. Elevated platelet-associated IgG in the thrombocytopenia of septicemia. N Engl J Med 300:760-4, 1979
  • Kelton JG, Neame PB, Bishop J, Ali M, Gauldie J, Hirsh J. The direct assay for platelet-associated IgG (PAIgG): lack of association between antibody level and platelet size. Blood 53:73-80, 1979