Heparin-Induced Thrombocytopenia (HIT)
Laboratory testing for investigation of HIT requires confirmation that the antibody will activate platelets in a heparin-dependent manner using a washed-platelet functional assay. The Serotonin Release Assay (SRA) developed in the McMaster Platelet Immunology Laboratory is the gold standard for the diagnosis of heparin‐induced thrombocytopenia1. The SRA is a functional test that detects pathologic HIT antibodies with high sensitivity and specificity. The McMaster Platelet Immunology laboratory provides HIT testing using a comprehensive investigation using the Serotonin Release Assay and the anti‐ PF4/heparin EIA.
Serotonin Release Assay (SRA) and PF4/heparin EIA
Information and Form:
- Specimen collection: Heparin-Induced Thrombocytopenia (HIT) Sample Requirments
- Patient and Test Requisition form
- Sheridan D, Carter C, Kelton JG. A diagnostic test for heparin-induced thrombocytopenia. Blood. 1986.