Research is focused on the molecular mechanisms of coagulation and fibrinolysis with emphasis on the clinical significance of findings.
Specific topics currently under investigation include:
- Mechanism of action of plasminogen activators
- Structure-activity relationships of coagulation enzymes, and their inhibitors,
- Mechanisms of action and potential clinical utility of new antithrombotic and thrombolytic agents
- Clinical trials investigating optimal methods for the diagnosis and treatment of venous and arterial thrombosis
Light scattering, fluorescence spectroscopy, and plasmon resonance are employed to study protein/protein, protein/carbohydrate or protein/DNA interactions. Kinetic analyses are used to examine rates of enzyme inactivation, whereas molecular biologic techniques (site-directed mutagenesis and expression of recombinant proteins in bacterial or cultured mammalian cell systems) are used to investigate structure-activity relationships of clotting enzymes and inhibitors. Hypotheses that originate from the results of basic research are then tested in animal models of arterial and venous thrombosis or in humans.