Investigations of normal and abnormal development of the extrinsic innervation of the gut
My research is focused on determining how, during ontogeny, vagal and sacral nerves find their targets in the bowel and on examining whether developmental abnormalities of this innervation contribute to the pathogenesis of disorders of gastrointestinal motility.
These studies employ a variety of techniques in molecular biology, including:
- Polymerase chain reaction (PCR)
- Reverse transcription PCR (RT-PCR)
- Quantitative PCR and in situ hybridization
Histological methods include:
- Tissue sectioning
- Microscopy (light and fluorescence)
- Image analysis.
Experiments in tissue culture involve maintenance of cell lines and primary cultures of explanted tissue. Animal work includes in-bred strains and several lines of transgenic mice. I am a member of the Farncombe Family Digestive Health Research Institute and collaborate with members of the Brain-Body Institute (BBI).