Molecular neuroendocrinology and neuropharmacology. Cellular and molecular mechanisms underlying drug-induced neuroprotection in animal models of neurodegeneration.
Current research projects include:
- Neurotrophic factor (eg GDNF, BDNF, NGF) induction by the neuroprotective hormones and drugs
- Stem cell transplantation and drug administration in neurotoxin models of Parkinson's disease
- Epigenetic modulation of gene expression in glial and neuronal cell lines by hormones, neurotransmitters and psychotropic drugs
Pharmacological, immunohistochemical and molecular studies in brain, cultured stem cells, neuronal and glial cell lines. Stereotaxic lesioning and neural stem cell transplantation in the brain. Examination of mRNA expression by RT-PCR and in situ hybridization. Confirmation of target gene expression by sequencing. Examination of protein expression by Western blotting, enzyme activity assays, immunohistochemistry and receptor binding assays. Reporter gene assays of promoter activity.