Denis P. Snider

, PhD, FCACB

Professor, Pathology and Molecular Medicine

Division: Clinical Pathology

Director, McMaster Flow Cytometry Facility
Head of Service, Histocompatibility Laboratory

McMaster University
3N26D Health Sciences Centre
905-525-9140 ext. 22813
sniderd@mcmaster.ca

Education and Professional Standing

• D(ABHI), 2004
• FCACB, 1995
• PhD Immunology, University of Toronto, 1986

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Interests

Research Focus

Mucosal Antibody Function and Measurement in Humans and Aspects of the anti-HLA Response in Transplantation.

Our current research concerns measurements of IgA antibody response in humans and aspects of anti-HLA antibodies in kidney transplantation. We use flow cytometry, ELISA, ELISPOT, in vitro B lymphocyte differentiation and culture, and multi-bead fluorescent analytic technologies to study human antibody responses.

Specific Areas

Antibody and T cell anti-HLA responses in kidney transplantation.

Immune function methodologies in clinical immunology.

Memory B cell and activate pre-plasma cells in human blood that secrete mucosal antibodies.

Methodologies

B cell functional assays, such as proliferation, differentiation and antibody production are performed to measure response to vaccines or infection. Cell receptor, intracelluar and surface molecule expression, and cell activation are all examined by flow cytometry. Human lymphocyte subsets are isolated from blood by rosette, magnetic bead or FACS. Molecular techniques include RT-PCR. Antibody responses are measured by ELISA, ELISPOT and multiplexed bead technologies, developed in the laboratory.

Other Research Roles

Dr. Snider is also the Director of the McMaster Flow Cytometry Facility. 

Clinical Focus

Dr. Snider’s clinical work includes:

• Staff Scientist; HRLMP, HHS
• Head of Service, Histocompatibility Lab; HRLMP, HHS

Academic Interests

Dr. Snider acts as a supervisor to Health Sciences Graduate Students and he heads committees in this area.  He also teaches residents during their lab medicine rotations for the Postgraduate MD Program.  Finally, Dr. Snider is involved in giving Immunology lectures at the Undergraduate level. 

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Team Members

Technicians: Hong Liang (Flow Cytometry Facility)

Graduate Students: Jason Fernandes

Selected Publications

• Pengfei Zhou, Rajka Borojevic, Cathy Streutker, Denis Snider, Hong Liang, Kenneth Croitoru. Expression of dual TcR on DO11.10 T cells allows for ovalbumen-induced oral tolerance to prevent cell-mediated colitis directed against unrelated enteric bacterial antigens. J. Immunol, 172:1515-1523. 2004.
• K Matsumoto, S Narita, T Rerecich, DP Snider, PM O’Byrne. Different profile of interleukin-10 production in circulating T cells from atopic asthmatics compared with healthy subjects. Can Respir J, 11(1):33-38. 2004.
• Krishnan Parameswaran, Hong Liang, Adrian Fanat, Richard Watson, Denis P. Snider, Paul M. O’Byrne. Role for cysteinyl leukotrienes in allergen-induced change in circulating dendritic cell number in asthma. J. Allergy Clin Immunol. 114(1):73-79, 2004.
• BW Timmons, MA Tarnoplolsky, DP Snider, and O Bar-Or. ImmunologicalChanges in Response to Exercise: Influence of Age, Puberty, and Gender. Med Sci Sports Exerc, 38(2):293-304, 2006.
• BW Timmons, MA Tarnopolsky, DP Snider, and O Bar-Or. Puberty Effects on NK Cell Responses to Exercise and Carbohydrate Intake in Boys. Med Sci Sports Exerc, 38(5):864-874, 2006.
• S Bhavanam, DP Snider, C Kaushic. Intranasal and subcutaneous immunization under the effect of estradiol leads to better protection against genital HSV-2 challenge compared to progesterone. Vaccine 26:6165-6172, 2008.

PubMed listing for Denis Snider