Mark WC Hatton

, PhD

Professor Emeritus
Pathology and Molecular Medicine

3N26G Health Sciences Centre
McMaster University
905-525-9140 ext. 22371
hattonm@mcmaster.ca

Education and Professional Standing

• Ph.D. University of London (UK) 1972
• M.Sc. University of London (UK) 1967
• B.Sc. University of London (UK) 1964

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Interests

Research Focus

My group is comparing the dynamics of hemostasis in an intravascular model of coagulation (i.e. the acutely-de-endothelialized aorta wall) and in an extravascular model (i.e. the procoagulant VX-2 lung tumor) in rabbits. For this purpose, interactions between individual blood proteins and platelets at the site of arterial injury or within the VX-2 tumor stroma are measured in vitro and in vivo. Using the VX-2 tumour model, the effusion of hemostatic factors (and their metabolic products) from the tumour stroma to the interpleural space in vivo is also of interest. These observations in interpleural effusates are currently being compared to ascites fluid obtained from cancer patients.

Rabbit proteins (and their products of catabolism) of past and present interest include: fibrinogen; prothrombin (thrombin), antithrombin, heparin cofactor II; factors VII, IX, X, XIII; plasminogen (plasmin; angiostatin), a2-antiplasmin; fibronectin, vitronectin and von Willebrand factor. The ultimate objective is to construct and compare models of hemostatic events that take place after arterial injury/healing, and during tumor growth.

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Team Members

Bonnie Ross and Suzanne Southward are able and accomplished Research Assistants who work with me. Narveen Jandu is a graduate student in my lab. Arlene Scopaz runs an efficient office for me and three faculty colleagues. To accomplish this (or any) research, it is fruitful (indeed essential!) to collaborate with colleagues in other disciplines.

I would also like to acknowledge the great participation I am currently receiving from the laboratories of Drs. Bryan Clarke, Bill Sheffield and Mo Blajchman of the Department of Pathology & Molecular Medicine (McMaster University), Drs. Mary Richardson, Gurmit Singh and Hal Hirte of the Research Group at the Hamilton Regional, and from Dr. Laszlo Bajzar of the Thrombosis Research Centre at the Henderson Hospital in Hamilton. Without help from these and many other colleagues in the past, much of what I do would not be conceivable let alone possible.

Selected Publications

• Hatton MWC, Southward SMR, Ross B, Legault K, Marien L, Korbie D, Richardson M, Singh G, Clarke BJ, Blajchman MA. Angiostatin-II is the predominant glycoform in pleural effusates of rabbit VX-2 lung tumors. J Lab Clin Med 2002; 139: 316-323.
• Hatton MWC, Day S, Southward SMR, DeReske M, Ross B, Seidlitz E, Singh G, Richardson, M. Metabolism of rabbit angiostatin glycoforms I and II in rabbits: Angiostatin-I leaves the intravascular space faster and appears to have greater anti-angiogenic activity than angiostatin-II. J Lab Clin Med 2001; 138: 83-93.
• Hatton MWC, Ross B, Southward SMR, DeReske M, Blajchman MA. The procoagulant state of the VX-2 tumor in rabbit lung in vivo: relative accumulation of fibrinogen, prothrombin, plasminogen, antithrombin and heparin cofactor II within the tumor. Thrombosis & Haemostasis 1999; 82: 1694-1702.
• Hatton MWC, Day S, Ross B, Southward SMR, DeReske M, Richardson M. Plasminogen-II accumulates five times faster than plasminogen-I at the site of a balloon de-endothelializing injury in vivo to the rabbit aorta. J Lab Clin Med 1999; 134 : 260-266.
• Hatton MWC, Ross B, Southward SMR, DeReske M, Richardson M: Pretreatment with Hirudin or Ancrod or Warfarin decreases the adsorption of fibrinogen and platelets by the de-endothelialized rabbit aorta wall immediately after balloon catheter injury in vivo. Arterioscler Thromb Vasc Biol 1998; 18: 816-824.
• Hatton MWC, Hoogendoorn H, Southward SMR, Ross B, Blajchman MA. Comparative metabolism and distribution of rabbit heparin cofactor-II and rabbit antithrombin in rabbits. Am J Physiol [Endocrin Metab 35] 1997; 272: E824-E831.
• Hatton MWC, Southward SMR, Ross-Ouellet B, DeReske M, Blajchman MA, Richardson M. An increased uptake of prothrombin, antithrombin and fibrinogen by the rabbit balloon-deendothelialized aorta surface in vivo is maintained until reendothelialization is complete. Arterioscler Thromb Vasc Biol 1996; 16: 1147-1155.
• Witmer M, Hatton MWC: Antithrombin III-ß associates more readily than -a with the uninjured and de-endothelialized rabbit aorta wall in vitro and in vivo. Arterioscler Thrombos 1991; 11: 530-539